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Cell line nameNeuro-2A
Accession numberICLC ATL99007
Brief descriptionSpecies: mouse, albino; Tumor: neuroblastoma
DescriptionSpecies: mouse, albino; Tumor: neuroblastoma
DepositorDr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy
Reference paperJ. Cell Biol. 1969, 43:69A; Proc: Natl Acad. Sci. USA 1970, 65:129-136
Morphology and growthmonoclonal continuous culture, grown as monolayer, morphology neuronal-like and round, mostly loosely attached cells, some in suspension
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split subconfluent cultures 1:3-1:6; seed at 2-4 x 104 cells/cm2; 5% CO2, 37C
Propertiesthe cells are reported to produce large quantities of microtubular protein, which plays a role in a contractile system which is responsible for axoplasmic flow in nerve cells
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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