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Cell line nameB104
Accession numberICLC ATL99008
Brief descriptionSpecies: rat, BDIX; Tissue: brain; Tumor: neuroblastoma
DescriptionSpecies: rat, BDIX adult; Tissue: brain; Tumor: neuroblastoma
DepositorDr F. Tosetti, Molecular Biology Dept IST at ABC, Genoa, Italy
Reference paperNature 1974;249:224-227 - DOI: 10.1038/249224a0 - PMID: 4151463
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast and neuroblast, small cells
Culture conditionsDMEM (4,5 g/L) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; split confluent cultures 1:10-1:20 using trypsin/EDTA (2-3 min.); seed at 1-3 x 104 cells/cm2; 5% CO
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyBrain Res 1981;254:563-575 - DOI: 10.1016/0165-3806(81)90024-9 - PMID: 6458347
J Neurochem 1999;72:2286-2293 - DOI: 10.1046/j.1471-4159.1999.0722286.x - PMID: 10349837
Proc Natl Acad Sci USA 1979;76:514-517 - DOI: 10.1073/pnas.76.1.514 - PMID: 284369

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