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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | B16-F10 |
| Accession number | ICLC ATL99010 |
| Brief description | Species: mouse, C57BL/6; Tumor: melanoma |
| Description | Species: mouse, C57BL/6; Tumor: melanoma |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | Cancer Res 1975;35:218-224 - PMID: 1109790 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | The cells produce melanin |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with NP, PepB |
| Tumorigenicity | tumorigenic and metastatic in syngeneic animals |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Nature 1973;242:148-149 |