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Cell line nameWOP
Accession numberICLC ATL99011
Brief descriptionSpecies: mouse; Tissue: embryo; Transformed by: polyoma virusreplication defective polyoma virus; Original line: 3T3
DescriptionSpecies: mouse; Tissue: embryo; Transformed by: polyoma virusreplication defective polyoma virus; Original line: 3T3
DepositorDr Ugo Borello, Hystology and Medical Embryology Dept, University of Rome La Sapienza, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with NP, PepB
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Virol 1985;54:739-749

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