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Interlab Cell Line Collection (ICLC)
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Cell line nameCEINGE CLONE 3
Accession numberICLC ATL99012
Brief descriptionSpecies: rat, Sprague-Dawley; Tissue: brain; Transformed by: murine leukemia virusmiddle T
DescriptionSpecies: rat, Sprague-Dawley female 2 week embryo; Tissue: brain; Transformed by: murine leukemia virusmiddle T
DepositorProf. T. Russo, Biochemistry and Medical Biotechnology Dept, Naples, Italy
Reference paperNeurosci Lett 1993;159:159-162 - DOI: 10.1016/0304-3940(93)90881-k - PMID: 8152593
Morphology and growthcontinuous culture, grown as monolayer, morphology oligodendrocyte
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available upon consent of the depositor. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with NP, MD, MPI
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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