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Cell line nameIST-EBV-TW5A
Accession numberICLC EBV03009
Brief descriptionSpecies: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DescriptionSpecies: human male 77 year old; Tissue: lymphocyte, B; Transformed by: Epstein-Barr; Derived from: peripheral blood
DepositorCell line established at ICLC cell bank. Sample provided by the Rome Twin Register
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast, cells growing as single cells or in clusters, some adherent
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 3-9 x 100.000 cells/ml; split 1:4-1:8; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle as Biosafety Level 2 containment
Passage number4
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsDizygous twin of IST-EBV-TW5B

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