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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HaCaT |
| Accession number | ICLC HL14001 |
| Brief description | Species: human; Tissue: skin |
| Description | Species: human; Tissue: skin; Derived from: adult human skin |
| Depositor | Dr. G. Angelini, IRCCS AOU San Martino - IST, Genoa, Italy |
| Reference paper | J Cell Biol. 1988 Mar;106(3):761-71 - DOI: 10.1083/jcb.106.3.761 - PMID: 2450098 |
| Morphology and growth | continuous culture, grown as monolayer, morphology Keratinocyte |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5 -1:8 using trypsin/EDTA (6 -10 min.); 5% CO2 |
| Properties | studying regulation of keratinization in human cells |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 1 containment |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. D5S818:11,12; D13S317:10,12; D7S820:9,11; D16S539:9,12; VWA:16,17; TH01:9.3; AM:X; TPOX:11,12; CSF1PO:9,11; D21S11:30.2 |
| Comments | Originator of the cell line: Petra Boukamp. In vitro spontaneous transformation of human adult keratinocytes from normal skin. |