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Cell line nameMRC-5
Accession numberICLC HL95001
Brief descriptionSpecies: human; Tissue: lung, fetal
DescriptionSpecies: human male 14 weeks old; Tissue: lung, fetal
DepositorObtained from ECACC.
Reference paperNature 1970;227:168-170 - DOI: 10.1038/227168a0 - PMID: 4316953
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
Propertiesvaccine development; senescence; virology; Susceptible to: poliovirus 1, herpes simplex, vesic. stomatitis (Indiana)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Passage number21
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 15; FGA: 21,23; TH01: 8; D18S51: 15,21; D21S11: 31.2; D8S1179: 13
Further bibliographyNature 1972;238:26 - DOI: 10.1038/239026a0 - PMID: 4342691
Nature 1972;239:316 - DOI: 10.1016/0006-291x(75)90293-4

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