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Interlab Cell Line Collection (ICLC)
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Cell line nameHFFF2
Accession numberICLC HL95002
Brief descriptionSpecies: human, Caucasian; Tissue: foreskin, fetal
DescriptionSpecies: human, Caucasian 14-18 weeks old; Tissue: foreskin, fetal
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-3x104 cells/cm2; 37C, 5% CO2. The cells grow very slowly.
Propertiesvirology; Susceptible to: cytomegalovirus, herpes simplex
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, MPI, NP
Validated by HLA-DP beta: confirmed as human by PCR
Passage number14
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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