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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NCTC 2544 |
| Accession number | ICLC HL97002 |
| Brief description | Species: human; Tissue: skin, keratinocyte |
| Description | Species: human; Tissue: skin, keratinocyte |
| Depositor | Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy. |
| Reference paper | Arch Dermatol Res 1976;256(3):255-260 - DOI: 10.1007/BF00572490 - PMID: 990102 |
| Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. Doubling time: 21 hours. |
| Properties | The cells show a good expression of cytochrome P450-dependent enzymatic activities, and could be a good cellular model for cytotoxicity studies; toxicology. |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Arch Dermatol Res 1976;261(1):27-31 - DOI: 10.1016/0006-291x(75)90293-4 |