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Cell line nameHCT-15
Accession numberICLC HTL00001
Brief descriptionSpecies: human; Tissue: colon; Tumor: colorectal adenocarcinoma
DescriptionSpecies: human; Tissue: colon; Tumor: colorectal adenocarcinoma
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1979;39:1020-1025

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