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Cell line nameRAJI
Accession numberICLC HTL00002
Brief descriptionSpecies: human, Black; Tumor: lymphoma, Burkitt
DescriptionSpecies: human, Black male 11 years old; Tumor: lymphoma, Burkitt
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperLancet 1964;1:238-240
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like, round cells, single and in clumps
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 0.4-1 x 10^6 cells/ml; split 1:5-1:10; 37C, 5% CO2
PropertiesEBNA positive
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 16,19,18; FGA: 19,27; TH01: 6,7; D18S51: 17; D21S11: 28,31; D8S1179: 14,15
Further bibliographyJ Exp Med 1976;143:1528-1533 - PMID: 1083890
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Trans N Y Acad Sci 1966;29:71 - PMID: 5232223
CommentsAfter thawing the cells look suffering; thaw with medium supplemented with 20% FBS

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