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IRCCS Ospedale Policlinico San Martino		 Interlab Cell Line Collection (ICLC)
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Cell line nameWiDr
Accession numberICLC HTL00003
Brief descriptionSpecies: human; Tissue: colon; Tumor: colorectal adenocarcinoma
DescriptionSpecies: human female 44 years old; Tissue: colon; Tumor: colorectal adenocarcinoma
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1974;34:105-108 - PMID: 4203458
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesProducts: carcinoembryonic antigen (CEA), colon specific antigen (CSAp), transforming growth factor beta, keratin; epidermal growth factor (EGF) receptor and p53 antigen expression. The cells are negative for Colon Antigen 3 expression. HLA: A24, A32; B15, B18
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyIn Vitro 1979;15:401-408 - DOI: 10.1007/BF02618407 - PMID: 90012
Proc Natl Acad Sci USA 1990;87:7555-7559 - DOI: 10.1073/pnas.87.19.7555 - PMID: 1699228
Science 1985;230:943-945 - DOI: 10.1126/science.3933111 - PMID: 3933111
CommentsShort tandem repeat (STR) genetic profiling has shown this cell line to be a derivative of HT-29 cell line.
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