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Cell line nameBT-474
Accession numberICLC HTL00008
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: carcinoma, ductal
DescriptionSpecies: human, Caucasian female 60 years old; Tissue: breast; Tumor: carcinoma, ductal
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperJ Natl Cancer Inst 1978;61:967-978 - PMID: 79029663
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial, compact, multi-layered colonies, rarely become confluent
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 0,01 mg/ml Bovine insulin; split cultures 1:2-1:3 using trypsin/EDTA (about 10 min.); 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Tumorigenicitytumorigenic in nude mice
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyIn Vitro 1979;15:723-729 - PMID: 80136092
CommentsThe cells grow slowly

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