ICLC logo Cell bank
IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameIMR-5
Accession numberICLC HTL00009
Brief descriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DepositorDr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblastic-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 (trypsin is not necessary); 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Biochem Biophys 1988,10:67-76 - PMID: 2906275
Cell Adhes Commun 2000;7:423-440 - DOI: 10.3109/15419060009109023 - PMID: 10830620
J Biol Chem 1995;270:1881-1887 - DOI: 10.1074/jbc.270.4.1881 - PMID: 7829525
CommentsThe viability can be low after thawing; thaw with medium supplemented with 20% FBS

For any info contact the Interlab Cell Line Collection
HTML by Paolo Romano