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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IMR-5 |
| Accession number | ICLC HTL00009 |
| Brief description | Species: human, Caucasian; Tumor: neuroblastoma |
| Description | Species: human, Caucasian; Tumor: neuroblastoma |
| Depositor | Dr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroblastic-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 (trypsin is not necessary); 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Biochem Biophys 1988,10:67-76 - PMID: 2906275
Cell Adhes Commun 2000;7:423-440 - DOI: 10.3109/15419060009109023 - PMID: 10830620 J Biol Chem 1995;270:1881-1887 - DOI: 10.1074/jbc.270.4.1881 - PMID: 7829525 |
| Comments | The viability can be low after thawing; thaw with medium supplemented with 20% FBS |