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Cell line nameIMR-5
Accession numberICLC HTL00009
Brief descriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DepositorDr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblastic-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 (trypsin is not necessary); 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Biochem Biophys 1988,10:67-76 - PMID: 2906275
Cell Adhes Commun 2000;7:423-440 - PMID: 10830620
J Biol Chem 1995;270:1881-1887 - PMID: 7829525
CommentsThe viability can be low after thawing; thaw with medium supplemented with 20% FBS

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