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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | COLO 205 |
| Accession number | ICLC HTL00010 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: colorectal adenocarcinoma |
| Description | Species: human, Caucasian male 70 years old; Tissue: colon; Tumor: colorectal adenocarcinoma; Derived from: ascitic fluid |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | Cancer Res 1978;38:1345-1355 - PMID: 565251 |
| Morphology and growth | continuous culture, grown semiadherent, morphology epithelial, many round cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:3-1:8 using trypsin/EDTA (2-3 min.); 37C, 5% CO2 |
| Properties | The cells are positive for keratin; products: carcinoembryonic antigen (CEA), interleukin 10 (IL-10) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Int J Cancer 1993;55:96-101 - DOI: 10.1002/ijc.2910550118
- PMID: 8344757
J Biol Chem 1993;268:24232-24241 - PMID: 7693697 |
| Comments | The cells grow loosely attached and in suspension |