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Cell line nameCOLO 205
Accession numberICLC HTL00010
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: colorectal adenocarcinoma
DescriptionSpecies: human, Caucasian male 70 years old; Tissue: colon; Tumor: colorectal adenocarcinoma; Derived from: ascitic fluid
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1978;38:1345-1355 - PMID: 565251
Morphology and growthcontinuous culture, grown semiadherent, morphology epithelial, many round cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:3-1:8 using trypsin/EDTA (2-3 min.); 37C, 5% CO2
PropertiesThe cells are positive for keratin; products: carcinoembryonic antigen (CEA), interleukin 10 (IL-10)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInt J Cancer 1993;55:96-101 - PMID: 8344757
J Biol Chem 1993;268:24232-24241 - PMID: 7693697
CommentsThe cells grow loosely attached and in suspension

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