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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | U87 MG |
| Accession number | ICLC HTL00013 |
| Brief description | Species: human, Caucasian; Tissue: brain; Tumor: glioblastoma-astrocytoma |
| Description | Species: human, Caucasian female 44 years old; Tissue: brain; Tumor: glioblastoma-astrocytoma |
| Depositor | Dr G. Tonini, Translational Pediatric Oncology Dept, IST at ABC, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 1mM Na pyruvate + 0.1mM non-essential amino acids; 37C, 5% CO2, split confluent culture 1:3 - 1:5 using trypsin/EDTA |
| Distribution | Cell line available for distribution |
| Hazard | Cell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment (Italian Decree Law 626/94) |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |