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Cell line nameU87 MG
Accession numberICLC HTL00013
Brief descriptionSpecies: human, Caucasian; Tissue: brain; Tumor: glioblastoma-astrocytoma
DescriptionSpecies: human, Caucasian female 44 years old; Tissue: brain; Tumor: glioblastoma-astrocytoma
DepositorDr G. Tonini, Translational Pediatric Oncology Dept, IST at ABC, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 1mM Na pyruvate + 0.1mM non-essential amino acids; 37C, 5% CO2, split confluent culture 1:3 - 1:5 using trypsin/EDTA
DistributionCell line available for distribution
HazardCell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment (Italian Decree Law 626/94)
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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