ICLC logo Cell bank
IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameSaos-2
Accession numberICLC HTL01001
Brief descriptionSpecies: human, Caucasian; Tissue: bone; Tumor: osteosarcoma
DescriptionSpecies: human, Caucasian female 11 years old; Tissue: bone; Tumor: osteosarcoma
DepositorDr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy
Reference paperHuman tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA, 37C, 5% CO2
Propertiesepidermal growth factor (EGF) and transforming growth factor beta (type 1 and type 2) receptor expression; positive for alkaline phosphatase
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number15
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X; vWA: 18; FGA: 22,25; TH01: 6,9; D18S51: 15; D21S11: 28,30; D8S1179: 10,12
Further bibliographyCancer Res. 1987;47:4961-4966 - PMID: 3040234
J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080

For any info contact the Interlab Cell Line Collection
HTML by Paolo Romano