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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | JURKAT |
| Accession number | ICLC HTL01002 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, T cell |
| Description | Species: human male 14 years old; Tissue: peripheral blood; Tumor: leukemia, T cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) |
| Depositor | Dr S. Stramigioli, Centro Trapianti Midollo Osseo, Pesaro, Italy. |
| Reference paper | Int J Cancer 1977;19:521-526 - DOI: 10.1002/ijc.2910190505 - PMID: 68013 |
| Morphology and growth | continuous culture, grown in suspension, morphology round cells , single or in clumps |
| Culture conditions | RPMI 1640 + 2 mM L-Glutamine + 15% FBS; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |