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Cell line nameALL-PO
Accession numberICLC HTL01003
Brief descriptionSpecies: human; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic
DescriptionSpecies: human female 4 months old; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic
DepositorDr A. Biondi, Clinica Pediatrica, Universita' Milano Bicocca, Monza, Italy
Reference paperLeuk Res 1997;21:1107-1114 - PMID: 9444945
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblastic, round, single cells
Culture conditionsRPMI 1640 + 10% FBS; maintain cultures between 0.5-1 x 10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyologyt(4;11) translocation
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThaw the cells with medium supplemented with 20% FBS.

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