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Cell line name1301
Accession numberICLC HTL01004
Brief descriptionSpecies: human, Caucasian; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell
DescriptionSpecies: human, Caucasian female 4 years old; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell; Derived from: CCRF-CEM
DepositorDAKO S.p.A., Milan, Italy; DAKO A/S, Glostrup, Denmark
Reference paperDev Biol Stand 1979;42:193-197 - PMID: 223921
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblastic, cells growing single and in clusters
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 2 and 9x10^5 cells/ml; split 1:5-1:10 every 2 to 3 days; 37C, 5% CO2
PropertiesThe cell line has very long telomeres
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyologyapprox. tetraploid
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyNucleic Acids Res. 1998;26(16):3651-6 - PMID: 9685479

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