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Cell line nameIST-MES2
Accession numberICLC HTL01007
Brief descriptionSpecies: human, Caucasian; Tissue: pleural effusion; Tumor: mesothelioma
DescriptionSpecies: human, Caucasian male 71 years old; Tissue: pleural effusion; Tumor: mesothelioma
DepositorDr A.M. Orengo, Immunopharmacology Dept, IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:3 using trypsin/EDTA; 37C, 5% CO2
PropertiesIL-6 and TGF-beta2 production. Tag protein expression in the nucleus. CAM5.2+; HBME-1+; LeuM1-; LeuM3-; LeuM6-;
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
KaryologyHypodiploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyEur Respir J 1999; 13:527-534 - PMID: 10232421
Int J Cancer 1998;78:740-749 - PMID: 9833768

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