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Cell line nameGDM-1
Accession numberICLC HTL01008
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, acute myelomonocytic
DescriptionSpecies: human female 66 years old; Tissue: peripheral blood; Tumor: leukemia, acute myelomonocytic
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperLeuk Res 1982;743-752 - DOI: 10.1016/0145-2126(82)90055-8 - PMID: 6296552
Morphology and growthcontinuous culture, grown in suspension, morphology single round to oval cells
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 5-8 x 10^5; split 1:3-1:5; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsCells grow slowly

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