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Cell line nameIST-MEL3
Accession numberICLC HTL01011
Brief descriptionSpecies: human, Caucasian; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human, Caucasian male adult; Tissue: skin; Tumor: melanoma
DepositorDr C. Semino, Immunoterapia Cellulare, IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like, some round cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:3 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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