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Cell line nameMDA-MB-453
Accession numberICLC HTL01013
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: carcinoma
DescriptionSpecies: human, Caucasian female 48 year old; Tissue: breast; Tumor: carcinoma; Derived from: pleural effusion
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperIn Vitro 1978;14:911-915 - PMID: 730202
Morphology and growthcontinuous culture, grown semiadherent, morphology epithelial, cells round in suspension
Culture conditionsL-15 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:4 using trypsin/EDTA; incubate at 37C without CO2 (close the flask caps)
PropertiesFibroblast growth factor (FGF) expression
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1979;39:919-922 - PMID: 427779
Cancer Res 1980;40:3118-3129 - PMID: 7000337
CommentsThe cells grow slowly

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