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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MDA-MB-453 |
| Accession number | ICLC HTL01013 |
| Brief description | Species: human, Caucasian; Tissue: breast; Tumor: carcinoma |
| Description | Species: human, Caucasian female 48 year old; Tissue: breast; Tumor: carcinoma; Derived from: pleural effusion |
| Depositor | Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy |
| Reference paper | In Vitro 1978;14:911-915 - DOI: 10.1007/BF02616120 - PMID: 730202 |
| Morphology and growth | continuous culture, grown semiadherent, morphology epithelial, cells round in suspension |
| Culture conditions | L-15 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:4 using trypsin/EDTA; incubate at 37C without CO2 (close the flask caps) |
| Properties | Fibroblast growth factor (FGF) expression |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1979;39:919-922 - PMID: 427779
Cancer Res 1980;40:3118-3129 - PMID: 7000337 |
| Comments | The cells grow slowly |