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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | SK-OV-3 |
Accession number | ICLC HTL01014 |
Brief description | Species: human, Caucasian; Tissue: ovary; Tumor: adenocarcinoma |
Description | Species: human, Caucasian female 64 year old; Tissue: ovary; Tumor: adenocarcinoma; Derived from: ascitic fluid |
Depositor | Dr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy |
Reference paper | Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp 155-159 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:6 using trypsin/EDTA; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Tumorigenicity | tumorigenic in nude mice |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
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