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Cell line nameMONO-MAC-6
Accession numberICLC HTL01015
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic
DescriptionSpecies: human male 64 year old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic
DepositorDr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Reference paperInt J Cancer 1988;41:456-461 - PMID: 3162233
Morphology and growthcontinuous culture, grown in suspension, morphology round, multiformed cells, some giant cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids + 9 ug/ml bovine insulin ; maintain cultures between 3x10^5 and 1x10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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