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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MONO-MAC-6 |
| Accession number | ICLC HTL01015 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
| Description | Species: human male 64 year old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
| Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy. |
| Reference paper | Int J Cancer 1988;41:456-461 - DOI: 10.1002/ijc.2910410324 - PMID: 3162233 |
| Morphology and growth | continuous culture, grown in suspension, morphology round, multiformed cells, some giant cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids + 9 ug/ml bovine insulin ; maintain cultures between 3x10^5 and 1x10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |