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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LS180 |
| Accession number | ICLC HTL01016 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma, colorectal |
| Description | Species: human, Caucasian female 58 year old; Tissue: colon; Tumor: adenocarcinoma, colorectal |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | In Vitro 1976;12:180-191 - DOI: 10.1007/BF02796440 - PMID: 1262041 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split cultures 1:4-1:6 using trypsin/EDTA (for at least 10 min.), or by scraping; 37C, 5% CO2 |
| Properties | CEA, IL-6, IL-10 and mucin production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | The cells are difficult to detach |