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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 5637 |
| Accession number | ICLC HTL01017 |
| Brief description | Species: human, Caucasian; Tissue: bladder; Tumor: carcinoma |
| Description | Species: human, Caucasian male 68 year old; Tissue: bladder; Tumor: carcinoma |
| Depositor | Dr M. Musso, Oncology, Biology and Genetic Dept, Genoa, Italy |
| Reference paper | Natl Cancer Inst Monogr 1978;49:5-9 - PMID: 571047 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:8 using trypsin/EDTA (8-10 min.); 37C, 5% CO2 |
| Properties | GM-CSF, IL-1, IL-6 production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 18; FGA: 22; TH01: 7,9; D18S51: --; D21S11: 36; D8S1179: 10,16 |
| Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
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