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Interlab Cell Line Collection (ICLC)
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Cell line nameMSTO-211H
Accession numberICLC HTL01018
Brief descriptionSpecies: human, Caucasian; Tumor: mesothelioma
DescriptionSpecies: human, Caucasian male 62 year old; Tumor: mesothelioma
DepositorDr L. Emionite, Preclinical Oncology Dept, CBA, Genoa, Italy
Reference paperDifferentiation 1988; 37:158-171 - DOI: 10.1111/j.1432-0436.1988.tb00806.x - PMID: 2840315
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardCell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment (Italian Decree Law 626/94).
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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