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Cell line nameCFPAC-1
Accession numberICLC HTL01019
Brief descriptionSpecies: human, Caucasian; Tissue: pancreas; Tumor: adenocarcinoma, ductal
DescriptionSpecies: human, Caucasian male 26 year old; Tissue: pancreas; Tumor: adenocarcinoma, ductal; Derived from: liver metastasis from a patient with cystic fibrosis
DepositorDr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Reference paperProc Natl Acad Sci USA 1990;87:4012-4016 - PMID: 90251687
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsIscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; split confluent cultures 1:3-1:6 using trypsin/EDTA (20 min.) or scraper; 37C, 5% CO2
PropertiesCEA production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers
CommentsThe cells are very difficult to detach and to disaggregate

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