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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-MEL-24 |
| Accession number | ICLC HTL02001 |
| Brief description | Species: human, Caucasian; Tissue: skin; Tumor: melanoma |
| Description | Species: human, Caucasian male 67 year old; Tissue: skin; Tumor: melanoma; Derived from: lymph node metastasis of a malignant melanoma |
| Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology stellate |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split 1:3-1:5 using trypsin/EDTA (10-15 min); 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 30 |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209
- PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080 |