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Cell line nameSK-MEL-24
Accession numberICLC HTL02001
Brief descriptionSpecies: human, Caucasian; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human, Caucasian male 67 year old; Tissue: skin; Tumor: melanoma; Derived from: lymph node metastasis of a malignant melanoma
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology stellate
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split 1:3-1:5 using trypsin/EDTA (10-15 min); 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number30
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

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