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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | C33A |
| Accession number | ICLC HTL02004 |
| Brief description | Species: human, Caucasian; Tissue: cervix; Tumor: carcinoma |
| Description | Species: human, Caucasian female 66 year old; Tissue: cervix; Tumor: carcinoma |
| Depositor | Dr L. Lanfranconi, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy |
| Reference paper | J Natl Cancer Inst 1964;32:135-148 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:8 using trypsin/EDTA; 37C, 5% CO2 |
| Properties | p53 expression, pRB (retinoblastoma protein) expression |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Am J Pathol 1985;119:361-366 - PMID: 2990217
Proc Natl Acad Sci USA 1991;88:5523-5527 - DOI: 10.1073/pnas.88.13.5523 - PMID: 1648218 |