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Cell line nameC33A
Accession numberICLC HTL02004
Brief descriptionSpecies: human, Caucasian; Tissue: cervix; Tumor: carcinoma
DescriptionSpecies: human, Caucasian female 66 year old; Tissue: cervix; Tumor: carcinoma
DepositorDr L. Lanfranconi, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperJ Natl Cancer Inst 1964;32:135-148
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:8 using trypsin/EDTA; 37C, 5% CO2
Propertiesp53 expression, pRB (retinoblastoma protein) expression
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Tumorigenicitytumorigenic in nude mice
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyAm J Pathol 1985;119:361-366 - PMID: 85248811
Proc Natl Acad Sci USA 1991;88:5523-5527 - PMID: 91288496

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