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Cell line nameMDA-MB-415
Accession numberICLC HTL02005
Brief descriptionSpecies: human; Tissue: breast; Tumor: adenocarcinoma
DescriptionSpecies: human female 38 year old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperIn Vitro 1978;14:911-915 - DOI: 10.1007/BF02616120 - PMID: 730202
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split cultures 1:3 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyCancer Biochem Biophys 1979;3:93-96 - PMID: 552902
Cancer Res 1979;39:919-922 - PMID: 427779
Cancer Res 1980;40:3118-3129 - PMID: 7000337

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