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Cell line nameBT-549
Accession numberICLC HTL02006
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: carcinoma, ductal
DescriptionSpecies: human, Caucasian female 72 year old; Tissue: breast; Tumor: carcinoma, ductal; Derived from: papillary, invasive ductal tumor
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial with multi-nucleated giant cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyCancer Res 1997;57:5386-5390 - PMID: 9393764
Cancer Res 1997;57:5441-5445 - PMID: 9407946

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