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Cell line nameA549
Accession numberICLC HTL03001
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: carcinoma
DescriptionSpecies: human, Caucasian male 58 year old; Tissue: lung; Tumor: carcinoma
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperJ Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37C, 5% CO2
PropertiesThe cells could synthesize lecithin and are positive for keratin
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyInt J Cancer 1976;17:62-70 - PMID: 175022

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