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Cell line nameHEL 92.1.7
Accession numberICLC HTL03002
Brief descriptionSpecies: human, Caucasian; Tumor: erythroleukemia
DescriptionSpecies: human, Caucasian male 30 year old; Tumor: erythroleukemia
DepositorObtained from Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy
Reference paperScience 1982;216:1233-1235 - DOI: 10.1126/science.6177045 - PMID: 6177045
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast, round, large and single cells. Some giant cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9 x 10^5 cells/ml; split 1:3-1:10; 37°C, 5% CO2
PropertiesUsed in the study of erythroid cell differentiation and globin gene expression
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
CommentsSome cells weakly adherent

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