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Cell line name293
Accession numberICLC HTL03003
Brief descriptionSpecies: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
DescriptionSpecies: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
DepositorObtained from ECACC
Reference paperJ Gen Virol 1977;36:59-74 - PMID: 886304
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2. The viability may be low after thawing and the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule and thawed with 20% FBS
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle as Biosafety Level 2 containment
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyVirology 1977;77:319-329 - PMID: 841862

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