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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 293 |
| Accession number | ICLC HTL03003 |
| Brief description | Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5 |
| Description | Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5 |
| Depositor | Obtained from ECACC |
| Reference paper | J Gen Virol 1977;36:59-74 - DOI: 10.1099/0022-1317-36-1-59 - PMID: 886304 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split cultures 1:3-1:6 using trypsin/EDTA; 37°C, 5% CO2. The viability may be low after thawing and the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule and thawed with 20% FBS |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle as Biosafety Level 2 containment |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Virology 1977;77:319-329 - DOI: 10.1016/0042-6822(77)90428-7
- PMID: 841862
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