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Cell line nameSK-BR-3
Accession numberICLC HTL03005
Brief descriptionSpecies: human; Tissue: breast; Tumor: adenocarcinoma
DescriptionSpecies: human female 43 year old; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion
DepositorProf. L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial, some round loosely attached cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split 1:2-1:4 using trypsin/EDTA; 37C, 5% CO2
PropertiesThe cell line overexpresses the HER/2c-erb-2 gene product
DistributionCell line available for distribution. For non-commercial investigative use only.
Hazard
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Recent Results Cancer Res 1976;57:33-41 - PMID: 1013510

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