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Cell line nameMDA-MB-435S
Accession numberICLC HTL03006
Brief descriptionSpecies: human; Tissue: breast; Tumor: carcinoma, ductal
DescriptionSpecies: human female 31 year old; Tissue: breast; Tumor: carcinoma, ductal; Derived from: metastatic site: pleural effusion
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperIn Vitro 1978;14:911-915 - PMID: 730202
Morphology and growthcontinuous culture, grown as monolayer, morphology spindle-shaped
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2
Propertiesproducts: tubulin, actin
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyCancer Res 1979;39:919-922 - PMID: 427779

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