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Cell line name293T
Accession numberICLC HTL04001
Brief descriptionSpecies: human; Tissue: kidney, embryonic; Transformed by: SV40large T antigen; Original line: 293
DescriptionSpecies: human; Tissue: kidney, embryonic; Transformed by: SV40large T antigen; Original line: 293
DepositorDr R. Notaro, Human Genetics Dept IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 20% FBS + 2mM L-Glutamine; Split subconfluent cultures (70% confluence)1:4-1:8 using trypsin/EDTA; 37C, 5% CO2
PropertiesSV40 large T antigen expression. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as 293tsA1609neo.
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyMol Cell Biol 1987;7:379-387 - PMID: 3031469
Proc Natl Acad Sci USA 1993;90:8392-8396 - PMID: 7690960
CommentsThe cells detach easily with PBS, but trypsin is necessary to disaggregate the cells

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