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Cell line nameM-07e
Accession numberICLC HTL04002
Brief descriptionSpecies: human, Caucasian; Tissue: peripheral blood; Tumor: leukemia, acute megakaryoblastic
DescriptionSpecies: human, Caucasian female 6 month old; Tissue: peripheral blood; Tumor: leukemia, acute megakaryoblastic; Derived from: M-07 growth factor-independent cell line
DepositorDr L. Pegoraro, University of Torino, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology single round cells and some small clusters, few cells are lightly adherent
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine + 10 ng/ml GM-CSF; Slit 1:2-1:3 every two days; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyBrit J Haematol 1988;69:359-366 - DOI: 10.1111/j.1365-2141.1988.tb02374.x - PMID: 3261598
J Cell Physiol 1990;145:458-464 - DOI: 10.1002/jcp.1041450310 - PMID: 2273055
Leukemia 1997;11:701-708 - DOI: 10.1038/sj.leu.2400633 - PMID: 9180295
CommentsThe viability may be low after thawing and need about one week to recover. The cells grow also with Iscove's MDM medium instead of RPMI and with IL-3 (10 ng/ml) instead of GM-CSF.

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