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Cell line nameHOS
Accession numberICLC HTL04003
Brief descriptionSpecies: human, Caucasian; Tissue: bone; Tumor: osteosarcoma
DescriptionSpecies: human, Caucasian female 13 year old; Tissue: bone; Tumor: osteosarcoma
DepositorProf. A. Gasperi-Campani, Sperimental Pathology Dept, University of Bologna, Italy
Reference paperCancer 1971;27:397-402 - PMID: 5100401
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like and epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split sub-confluent (70-80%) cultures 1:10-1:12 twice for week using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number48
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyInt J Cancer 1975;15:23-29 - PMID: 165148
Int J Cancer 1975;16:840-849 - PMID: 23195

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