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Cell line nameA-498
Accession numberICLC HTL04005
Brief descriptionSpecies: human; Tissue: kidney; Tumor: adenocarcinoma
DescriptionSpecies: human female 52 years old; Tissue: kidney; Tumor: adenocarcinoma
DepositorDr. M. Viale, Pharmacology Dept, IST, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; Split confluent cultures 1:3-1:6 three times a week using trypsin/EDTA (2-3 minutes); 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

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