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Cell line nameROV-S
Accession numberICLC HTL05002
Brief descriptionSpecies: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr
DescriptionSpecies: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr; Derived from: children with maturation defects of haematopoiesis
DepositorDr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology monocytic, round, single and small clusters
Culture conditionsRPMI 1640 + 20%FCS + 5ng/ml GM-CSF + 2mML-Glutamine; split 1:5, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number51
Freezing mediumCulture medium 50% + 40%FCS + 10%DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Gen Virol 1989;70:(5)1203-15 - PMID: 2543775

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