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Cell line nameLB-F9
Accession numberICLC HTL05004
Brief descriptionSpecies: human; Tissue: lymphocyte, B; Pathology: paroxysmal nocturnal hemoglobinuria; Transformed by: Epstein-Barr
DescriptionSpecies: human female; Tissue: lymphocyte, B; Pathology: paroxysmal nocturnal hemoglobinuria; Transformed by: Epstein-Barr
DepositorDr R. Notaro, Human Genetic Dept IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast, single and in small clusters growth
Culture conditionsRPMI 1640 + 4 mML-Glutamine + 20% FBS ; maintain cultures between 350-400 x 10^3 cells/ml; duplication time 48-60 h; 37C, 5% CO2
PropertiesGPI-
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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