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Cell line nameH9
Accession numberICLC HTL05006
Brief descriptionSpecies: human, Caucasian; Tissue: lymphocyte, T; Tumor: lymphoma
DescriptionSpecies: human, Caucasian male 53 years old; Tissue: lymphocyte, T; Tumor: lymphoma; Derived from: HuT 78
DepositorDr. A. De Maria, Internal Medicine Dept (DIMI), Genoa, Italy
Reference paperAIDS Res Hum Retroviruses 1989; 5(3):253-5 - PMID: 2567177
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like, cells growing single and in clusters
Culture conditionsRPMI 1640 + 10% FBS + Antibiotics + 2mM L-Glutamine; split 1:10 every 2 days; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyBlood 1980; 55:409-417 - PMID: 6244013
J Natl Cancer Inst 1992; 84:1922-1926 - PMID: 1460674
CommentsThaw the cells with medium supplemented with FBS 20%

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