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Cell line nameTHP-1 h
Accession numberICLC HTL05008
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic
DescriptionSpecies: human male 1 years old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic; Derived from: THP-1
DepositorDr A. De Lerma, Clinical and Biological Science Dept, Faculty of Medicine, Insubria University, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology large, round, single cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9x105 cells/ml; 37C, 5% CO2
PropertiesLow constitutive expression of HLA-II; high expression of HLA-II after treatment with IFNgamma.
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 16; FGA: 24,25; TH01: 8,9.3; D18S51: 13,14; D21S11: 30,31.2; D8S1179: 10,14; Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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