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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NCI-H1650 |
| Accession number | ICLC HTL06002 |
| Brief description | Species: human, Caucasian; Tissue: lung; Tumor: adenocarcinoma, bronchioalveolar carcinoma |
| Description | Species: human, Caucasian male 27 year old; Tissue: lung; Tumor: adenocarcinoma, bronchioalveolar carcinoma; Derived from: pleural effusion |
| Depositor | Dr P. Russo, Translational Oncology Dept, IST at ABC, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mML-Glutamine + 1mM Na pyruvate + 1,5 g/L Na bicarbonate; split confluent cultures1:4-1:6 using trypsin/EDTA; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only. |
| Hazard | |
| Freezing medium | Culture medium + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Science 2004;305(5687):1163-7. - DOI: 10.1126/science.1101637
- PMID: 15284455
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| Comments | smoker patient. |