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Cell line nameNCI-H1650
Accession numberICLC HTL06002
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: adenocarcinoma, bronchioalveolar carcinoma
DescriptionSpecies: human, Caucasian male 27 year old; Tissue: lung; Tumor: adenocarcinoma, bronchioalveolar carcinoma; Derived from: pleural effusion
DepositorDr P. Russo, Translational Oncology Dept, IST at ABC, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mML-Glutamine + 1mM Na pyruvate + 1,5 g/L Na bicarbonate; split confluent cultures1:4-1:6 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only.
Hazard
Freezing mediumCulture medium + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyScience 2004;305(5687):1163-7. - PMID: 15284455
Commentssmoker patient.

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