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Cell line nameCM-S/un
Accession numberICLC HTL06005
Brief descriptionSpecies: human, Caucasian; Tissue: bone marrow; Tumor: monocyte tumor
DescriptionSpecies: human, Caucasian; Tissue: bone marrow; Tumor: monocyte tumor; Derived from: patient with defects of haematopoiesis
DepositorDr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy
Reference paperCancer Res 1982;42(10):4182-9 - PMID: 7105012
Morphology and growthcontinuous culture, grown in suspension, morphology monocytic, round, single and small clusters
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:10-1:15, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number4
Freezing mediumCulture medium 50% + 40%FCS + 10%DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyCancer Res. 1985;45(3):1392-401 - PMID: 3971383
FEBS Lett. 1985 Sep 9;189(1):97-101 - PMID: 2993032
CommentsThawed with 20%FCS.

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